| Title | Generation of pluripotent stem cells from patients with type 1 diabetes. |
| Publication Type | Journal Article |
| Year of Publication | 2009 |
| Authors | Maehr R, Chen S, Snitow M, Ludwig T, Yagasaki L, Goland R, Leibel RL, Melton DA |
| Journal | Proc Natl Acad Sci U S A |
| Volume | 106 |
| Issue | 37 |
| Pagination | 15768-73 |
| Date Published | 2009 Sep 15 |
| ISSN | 1091-6490 |
| Keywords | Adult, Adult Stem Cells, Cell Dedifferentiation, Cell Differentiation, Cells, Cultured, Diabetes Mellitus, Type 1, Endoderm, Germ Layers, Humans, Kruppel-Like Factor 4, Male, Models, Biological, Pancreas, Pluripotent Stem Cells, Transcription Factors |
| Abstract | <p>Type 1 diabetes (T1D) is the result of an autoimmune destruction of pancreatic beta cells. The cellular and molecular defects that cause the disease remain unknown. Pluripotent cells generated from patients with T1D would be useful for disease modeling. We show here that induced pluripotent stem (iPS) cells can be generated from patients with T1D by reprogramming their adult fibroblasts with three transcription factors (OCT4, SOX2, KLF4). T1D-specific iPS cells, termed DiPS cells, have the hallmarks of pluripotency and can be differentiated into insulin-producing cells. These results are a step toward using DiPS cells in T1D disease modeling, as well as for cell replacement therapy.</p> |
| DOI | 10.1073/pnas.0906894106 |
| Alternate Journal | Proc Natl Acad Sci U S A |
| PubMed ID | 19720998 |
| PubMed Central ID | PMC2735559 |
| Grant List | R01 DK052431 / DK / NIDDK NIH HHS / United States R01 DK066518 / DK / NIDDK NIH HHS / United States / HHMI / Howard Hughes Medical Institute / United States |