Hartman Institute for Therapeutic Organ Regeneration

Stromal-derived factor-1 delivered via hydrogel drug-delivery vehicle accelerates wound healing in vivo.

TitleStromal-derived factor-1 delivered via hydrogel drug-delivery vehicle accelerates wound healing in vivo.
Publication TypeJournal Article
Year of Publication2011
AuthorsHenderson PW, Singh SP, Krijgh DD, Yamamoto M, Rafii DC, Sung JJ, Rafii S, Rabbany SY, Spector JA
JournalWound Repair Regen
Volume19
Issue3
Pagination420-5
Date Published2011 May-Jun
ISSN1524-475X
KeywordsAnimals, Antigens, CD, Cadherins, Cell Culture Techniques, Chemokine CXCL12, Drug Delivery Systems, Hydrogel, Polyethylene Glycol Dimethacrylate, Male, Mice, Mice, Inbred C57BL, Tissue Engineering, Tissue Scaffolds, von Willebrand Factor, Wound Healing
Abstract

<p>Topical treatment of superficial wounds has many advantages including decreased cost and increased ease of application compared with systemic treatments. Many of the advantages, however, are lost when it is necessary for repeated doses of topical medications to be given over an extended period of time. Therefore, a drug-delivery vehicle that delivers biologically appropriate doses in a sustained fashion would prove valuable. In this study, an alginate hydrogel scaffold impregnated with the angiogenic chemokine stromal-derived factor-1 was used to provide targeted, though short-term, delivery directly into the wound bed. Wounds were created on the dorsum of mice, and either a stromal-derived factor-1-impregnated or a saline-impregnated scaffold was applied. Wounds were explanted after 1, 3, 7 days, wound area was measured, and histology and immunohistochemistry for endothelial markers were performed. The remaining wound area in stromal-derived factor-1-treated wounds vs. controls was not significant 1 day after wounding (96.7 ± 0.1 vs. 97.5 ± 1.1%, p=0.317), but was significant after 3 days postwounding (46.7 ± 0.1 vs. 82.3 ± 2.4%, p=0.046) and 7 days postwounding (2.3 ± 1.3 vs. 32.0 ± 4.0%, p=0.049). Immunohistochemistry revealed a greater degree of endothelial cell invasion into the wound bed infiltration compared with controls. The results of this study suggest significant clinical promise for our hydrogel-delivery vehicle in the treatment of wounds.</p>

DOI10.1111/j.1524-475X.2011.00687.x
Alternate JournalWound Repair Regen
PubMed ID21518091
Grant List / / Howard Hughes Medical Institute / United States

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