Hartman Institute for Therapeutic Organ Regeneration

A promoter of the rat insulin-like growth factor II gene consists of minimal control elements.

TitleA promoter of the rat insulin-like growth factor II gene consists of minimal control elements.
Publication TypeJournal Article
Year of Publication1988
AuthorsEvans T, DeChiara T, Efstratiadis A
JournalJ Mol Biol
Volume199
Issue1
Pagination61-81
Date Published1988 Jan 05
ISSN0022-2836
KeywordsAnimals, Base Sequence, Binding Sites, Cells, Cultured, DNA, Genes, Insulin-Like Growth Factor II, Molecular Sequence Data, Mutation, Promoter Regions, Genetic, Rats, Somatomedins, Templates, Genetic, Transcription, Genetic
Abstract

We have characterized the cis-control signals in one of the two promoters of the developmentally regulated rat insulin-like growth factor II gene (rIGF-II) by a combination of in-vivo transient expression, in-vitro transcription, footprinting, gel band-shifting and methylation-interference experiments, using a series of deletion mutant templates. Our results indicate that this simple (minimal) promoter (P2) consists of no more than 128 base-pairs, which include an ATA box and four proximal upstream GC boxes binding the general transcription factor Sp1. Three of the latter sites deviate from the known Sp1 consensus recognition sequence. The two types of cis-acting regulatory signals (GC/ATA motif) of the P2 promoter are inter-dependent and sufficient for transcription. A model for the operation of this type of minimal promoter is discussed. S1 nuclease-hypersensitive sites, localized by in-vitro mapping to the region of the P2 Sp1-binding sites, are also present in vivo and correlate with the transcriptional state of chromatin in the rIGF-II locus. We show that recognition sites for Sp1 binding are a subset of sequences that exhibit hypersensitivity to S1.

DOI10.1016/0022-2836(88)90379-8
Alternate JournalJ Mol Biol
PubMed ID3351924

Weill Cornell Medicine
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