| Title | S1-hypersensitive sites in eukaryotic promoter regions. |
| Publication Type | Journal Article |
| Year of Publication | 1984 |
| Authors | Evans T, Schon E, Gora-Maslak G, Patterson J, Efstratiadis A |
| Journal | Nucleic Acids Res |
| Volume | 12 |
| Issue | 21 |
| Pagination | 8043-58 |
| Date Published | 1984 Nov 12 |
| ISSN | 0305-1048 |
| Keywords | Animals, DNA Restriction Enzymes, Endonucleases, Genes, Globins, Humans, Insulin, Plasmids, Proinsulin, Promoter Regions, Genetic, Protein Precursors, Rats, Simian virus 40, Single-Strand Specific DNA and RNA Endonucleases, Substrate Specificity |
| Abstract | We have examined by fine mapping the S1 nuclease-hypersensitivity of the 5' flanking regions of the human beta-globin and rat preproinsulin II genes and of the SV40 origin/enhancer region. In all cases S1-hypersensitive sites are located in known or presumed promoter/regulatory regions. Though a consensus DNA sequence is not evident, all of these sites reside in predominantly homopurine-homopyrimidine stretches. The alternate (non-B) DNA structure which is revealed by the enzymatic probe is a sequence-dependent feature of a short stretch of DNA, which is retained upon transplantation into a foreign environment. The alternate structure exhibits S1-nicking patterns uniquely different from those associated with the presence of Z-DNA. |
| DOI | 10.1093/nar/12.21.8043 |
| Alternate Journal | Nucleic Acids Res |
| PubMed ID | 6095186 |
| PubMed Central ID | PMC320272 |