| Title | Direct lineage conversion of pancreatic exocrine to endocrine Beta cells in vivo with defined factors. |
| Publication Type | Journal Article |
| Year of Publication | 2014 |
| Authors | Cavelti-Weder C, Li W, Weir GC, Zhou Q |
| Journal | Methods Mol Biol |
| Volume | 1150 |
| Pagination | 247-62 |
| Date Published | 2014 |
| ISSN | 1940-6029 |
| Keywords | Adenoviridae, Animals, Base Sequence, Cell Lineage, Cellular Reprogramming, Cloning, Molecular, Diabetes Mellitus, Insulin-Secreting Cells, Male, Mice, Oligonucleotides, Pancreas, Exocrine |
| Abstract | <p>Pancreatic exocrine cells can be directly converted to insulin(+) beta cells by adenoviral-mediated expression of three transcription factors Pdx1, Mafa, and Ngn3 in the adult mouse pancreas (Zhou et al., Nature 455(7213):627-632, 2008). This direct reprogramming approach offers a strategy to replenish beta-cell mass and may be further developed as a potential future treatment for diabetes. Here, we provide a detailed protocol for inducing exocrine to beta-cell reprogramming in mice. We also describe key analyses we routinely use to assess the phenotype and function of reprogrammed cells.</p> |
| DOI | 10.1007/978-1-4939-0512-6_17 |
| Alternate Journal | Methods Mol Biol |
| PubMed ID | 24744004 |
| Grant List | R00 DK077445 / DK / NIDDK NIH HHS / United States |