| Title | Isolation and Characterization of Mouse Organ-Specific Endothelial Transcriptomes. |
| Publication Type | Journal Article |
| Year of Publication | 2018 |
| Authors | Rafii S, Palikuqi B |
| Journal | Methods Mol Biol |
| Volume | 1846 |
| Pagination | 301-308 |
| Date Published | 2018 |
| ISSN | 1940-6029 |
| Keywords | Animals, Biomarkers, Cell Separation, Cells, Cultured, Endothelial Cells, Gene Expression Profiling, Immunohistochemistry, Mice, Organ Specificity, Transcriptome |
| Abstract | <p>Endothelial cells play an essential role in homeostasis and regeneration and their transcriptional profile has been found to be tissue specific. Here we describe the isolation of organ specific mouse endothelial cells using either magnetic beads or fluorescence-activated cell sorting. This efficient protocol should allow for the transcriptional characterization of organotypic endothelial cells.</p> |
| DOI | 10.1007/978-1-4939-8712-2_20 |
| Alternate Journal | Methods Mol Biol |
| PubMed ID | 30242768 |