| Title | Interaction of the erythroid transcription factor cGATA-1 with a critical auto-regulatory element. |
| Publication Type | Journal Article |
| Year of Publication | 1992 |
| Authors | Schwartzbauer G, Schlesinger K, Evans T |
| Journal | Nucleic Acids Res |
| Volume | 20 |
| Issue | 17 |
| Pagination | 4429-36 |
| Date Published | 1992 Sep 11 |
| ISSN | 0305-1048 |
| Keywords | Animals, Base Sequence, Binding Sites, Cells, Cultured, Chickens, Cloning, Molecular, Consensus Sequence, DNA, DNA Mutational Analysis, DNA-Binding Proteins, Erythrocytes, Erythroid-Specific DNA-Binding Factors, Molecular Sequence Data, Nucleic Acid Conformation, Plasmids, Promoter Regions, Genetic, Recombinant Fusion Proteins, Restriction Mapping, Transcription Factors, Transcription, Genetic |
| Abstract | We have performed a mutational analysis of the promoter for the chicken erythroid-specific GATA-1 transcription factor, and have investigated in detail the interaction of the factor with an upstream auto-regulatory element (ARE). We find that a single proximal GATA binding site of the ARE is required for promoter activity in primary erythroid cells; however, this minimal promoter is inappropriately active in fibroblasts. At least two molecules of GATA-1 can interact with the ARE, and sequences outside of the consensus site appear critical for the transcriptional activity of the bound protein. Finally, we provide evidence for complex protein/DNA interactions at the ARE, including the ability of GATA-1 to bend DNA. |
| DOI | 10.1093/nar/20.17.4429 |
| Alternate Journal | Nucleic Acids Res |
| PubMed ID | 1408744 |
| PubMed Central ID | PMC334168 |
| Grant List | DK44167 / DK / NIDDK NIH HHS / United States |