| Title | Genetic targeting of the endoderm with claudin-6CreER. |
| Publication Type | Journal Article |
| Year of Publication | 2008 |
| Authors | Anderson WJ, Zhou Q, Alcalde V, Kaneko OF, Blank LJ, Sherwood RI, J Guseh S, Rajagopal J, Melton DA |
| Journal | Dev Dyn |
| Volume | 237 |
| Issue | 2 |
| Pagination | 504-12 |
| Date Published | 2008 Feb |
| ISSN | 1058-8388 |
| Keywords | Animals, Cell Lineage, Claudins, DNA Primers, Endoderm, Galactosides, Gene Expression Regulation, Developmental, Genotype, In Situ Hybridization, Indoles, Integrases, Membrane Proteins, Mice, Mice, Knockout, Reverse Transcriptase Polymerase Chain Reaction |
| Abstract | <p>A full description of the ontogeny of the beta cell would guide efforts to generate beta cells from embryonic stem cells (ESCs). The first step requires an understanding of definitive endoderm: the genes and signals responsible for its specification, proliferation, and patterning. This report describes a global marker of definitive endoderm, Claudin-6 (Cldn6). We report its expression in early development with particular attention to definitive endoderm derivatives. To create a genetic system to drive gene expression throughout the definitive endoderm with both spatial and temporal control, we target the endogenous locus with an inducible Cre recombinase (Cre-ER(T2)) cassette. Cldn6 null mice are viable and fertile with no obvious phenotypic abnormalities. We also report a lineage analysis of the fate of Cldn6-expressing embryonic cells, which is relevant to the development of the pancreas, lung, and liver.</p> |
| DOI | 10.1002/dvdy.21437 |
| Alternate Journal | Dev Dyn |
| PubMed ID | 18213590 |
| PubMed Central ID | PMC2665265 |
| Grant List | K99 DK077445 / DK / NIDDK NIH HHS / United States / HHMI / Howard Hughes Medical Institute / United States 5U01DK072505-02 / DK / NIDDK NIH HHS / United States |