Title | BMP signaling restricts hemato-vascular development from lateral mesoderm during somitogenesis. |
Publication Type | Journal Article |
Year of Publication | 2006 |
Authors | Gupta S, Zhu H, Zon LI, Evans T |
Journal | Development |
Volume | 133 |
Issue | 11 |
Pagination | 2177-87 |
Date Published | 2006 Jun |
ISSN | 0950-1991 |
Keywords | Animals, Animals, Genetically Modified, Biomarkers, Bone Morphogenetic Protein Receptors, Bone Morphogenetic Proteins, Deoxyribonucleases, Type II Site-Specific, Gene Expression Regulation, Developmental, Hematopoiesis, Kidney, Mesoderm, PAX2 Transcription Factor, Saccharomyces cerevisiae Proteins, Signal Transduction, Somites, Stem Cells, Time Factors, Zebrafish, Zebrafish Proteins |
Abstract | The bone morphogenetic protein (BMP) signaling pathway is essential during gastrulation for the generation of ventral mesoderm, which makes it a challenge to define functions for this pathway at later stages of development. We have established an approach to disrupt BMP signaling specifically in lateral mesoderm during somitogenesis, by targeting a dominant-negative BMP receptor to Lmo2+ cells in developing zebrafish embryos. This results in expansion of hematopoietic and endothelial cells, while restricting the expression domain of the pronephric marker pax2.1. Expression of a constitutively active receptor and transplantation experiments were used to confirm that BMP signaling in lateral mesoderm restricts subsequent hemato-vascular development. The results show that the BMP signaling pathway continues to function after cells are committed to a lateral mesoderm fate, and influences subsequent lineage decisions by restricting hemato-vascular fate in favor of pronephric development. |
DOI | 10.1242/dev.02386 |
Alternate Journal | Development |
PubMed ID | 16672337 |
Grant List | HL056182 / HL / NHLBI NIH HHS / United States |