Hartman Institute for Therapeutic Organ Regeneration

BMP signaling restricts hemato-vascular development from lateral mesoderm during somitogenesis.

TitleBMP signaling restricts hemato-vascular development from lateral mesoderm during somitogenesis.
Publication TypeJournal Article
Year of Publication2006
AuthorsGupta S, Zhu H, Zon LI, Evans T
JournalDevelopment
Volume133
Issue11
Pagination2177-87
Date Published2006 Jun
ISSN0950-1991
KeywordsAnimals, Animals, Genetically Modified, Biomarkers, Bone Morphogenetic Protein Receptors, Bone Morphogenetic Proteins, Deoxyribonucleases, Type II Site-Specific, Gene Expression Regulation, Developmental, Hematopoiesis, Kidney, Mesoderm, PAX2 Transcription Factor, Saccharomyces cerevisiae Proteins, Signal Transduction, Somites, Stem Cells, Time Factors, Zebrafish, Zebrafish Proteins
Abstract

The bone morphogenetic protein (BMP) signaling pathway is essential during gastrulation for the generation of ventral mesoderm, which makes it a challenge to define functions for this pathway at later stages of development. We have established an approach to disrupt BMP signaling specifically in lateral mesoderm during somitogenesis, by targeting a dominant-negative BMP receptor to Lmo2+ cells in developing zebrafish embryos. This results in expansion of hematopoietic and endothelial cells, while restricting the expression domain of the pronephric marker pax2.1. Expression of a constitutively active receptor and transplantation experiments were used to confirm that BMP signaling in lateral mesoderm restricts subsequent hemato-vascular development. The results show that the BMP signaling pathway continues to function after cells are committed to a lateral mesoderm fate, and influences subsequent lineage decisions by restricting hemato-vascular fate in favor of pronephric development.

DOI10.1242/dev.02386
Alternate JournalDevelopment
PubMed ID16672337
Grant ListHL056182 / HL / NHLBI NIH HHS / United States

Weill Cornell Medicine
Hartman Institute for Therapeutic Organ Regeneration
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